Efficient Experiment Tracking with Revvity Signals

In this document, we will guide you through a structured process to efficiently track and manage experiments using Revvity Signals Software. The steps outlined will help you leverage the advanced features of Signals Notebook to organize data, manage samples, and streamline workflows.

Step 1

This video demonstrates an overview of the bio method tracking example experiment we have prepared. In this example, we use structured data elements, such as admin-defined tables, equipment, material tables, and worksheets.

Step 2

To add samples, use the "Add Sample" menu option located on the left. When starting with your template, add content and samples through this menu option.

Step 3

We will review task plans and incorporate them into the experiment template. You'll learn how to import materials, equipment results, and information for an ADT that performs calculations for you. We will also cover how to integrate bioprocess methods, equipment, feeding schedules, and sample tables. Start by selecting "Add New Experiment" and choose a template.

Step 4

You may select any template to explore. For this guide, we will proceed with the bioprocess experiment and select our notebook.

Step 5

We can add additional fields by selecting "Project B" and creating our experiment. This action will import the designed template to begin building the required information for the bioprocess. Initially, there is a text box for input.

Step 6

Enter "Testing Experiment Organization" in the text box. For samples, use the "Add Content" button to include samples at the top of the page.

Step 7

Create the sample template for the bioprocess by selecting the desired number of samples and adding common property values.

Step 8

Select a material ID, which links to the materials available in our material libraries, and choose one accordingly.

Step 9

Add parent references by selecting from the material library options. For instance, select a plasmid as a material parent reference.

Step 10

Specify the required volumes and select a date. Adjust the time as needed and click "Create."

Step 11

The samples will be added to the sample table. You can now include other descriptions and enter the desired values, such as "two, four, six."

Step 12

All changes are saved automatically. Proceed to the task plan, where you can create tasks from "Add Content" or add tasks from the sample table, incorporating your samples.

Step 13

To create tasks, select the template task, choose the samples, and optionally assign an analyst or group to the task.

Step 14

Tasks are accessible to any user. Once created, you can quickly organize tasks by dragging and dropping them as needed.

Step 15

Select from customizable bioassays for your team. Each has a task ID, bioassay test, and sample references.

Step 16

Samples and tasks can be added to this plan. For materials, the cell line material library is automatically selected. Scan a barcode or enter the ID, or search for materials from the library.

Step 17

Add the selected cell line. If there's a container in inventory, select and confirm it, or switch containers as necessary.

Step 18

The material library is configured for inventory decrements. Enter the required amount for the experiment, which will automatically adjust the container total. This also applies to material cell pools.

Step 19

The material library can reference multiple items. Add selected materials, which will include references to the cell IDs within the pool. For analysis, upload data or import from CSV.

Step 20

Instrumentation can connect via APIs, external actions, or DLX. For this example, import from CSV, then select your instrument results file to import.

Step 21

Import the selected file, bringing in instrument data, run types, and additional equipment information. Add content at the top for various table types.

Step 22

To record ViaCell equipment results, select the cell viability table, which includes input data and auto-populated calculations. Experiment with these tables to see their functionality.

Step 23

Choose the cell viability table and click "Create." This action will load the table template, allowing you to record data for selected samples.

Step 24

With the information automatically saved, proceed to import ViaCell results for analysis.

Step 25

After importing results, check the initial cells and current end viability status. Confirm viability and hover over to view formulas. Record the current date.

Step 26

Select today's date for each sample, which can be automated. Specify the previous day to track the testing duration in hours.

Step 27

This process tracks testing duration. Monitor time changes and view relevant information, including growth rate and cell doubling time.

Step 28

Tables are customizable for various calculations and data inputs. The template mimics these functionalities, automatically populating conditions.

Step 29

Input the equipment details, automatically retrieving information such as serial number, manufacturer, usage date, and location. The calibrated date is also available if applicable.

Step 30

Integrate an additional ADT to track the bioreactor. Set start and run dates, check run steps, and auto-populate feed volume and temperature details.

Step 31

The sampling table allows volume input and performs calculations. Gray areas are auto-populated.

Step 32

Enter random numbers and select references, using recently created samples. Flags indicate whether conditions are met.

Step 33

Worksheets are embedded for testing and configuration, with a variety of options available for design.

Step 34

The top button allows you to add diverse content types. Files can be uploaded and content rearranged as necessary.